Helric Fredou, 45, shot himself in his office with his police-issue gun on Wednesday night in Limoges, a city in central France, according to France 3.
Commissioner Fredou began his career as a police office in 1997 and had been the deputy director of the regional police since 2012.
Brothers Cherif and Said Kouachi shot 12 people dead at the Charlie Hebdo offices on Wednesday before seeking refuge in a print works.
They were killed on Friday afternoon during a shoot-out with police, as was their alleged accomplice, Amedy Coulibaly, who killed four hostages at a kosher supermarket in Paris.
France's national police union has expressed its 'sincere condolences' over Commissioner Fredou's death and said they were thinking especially of his colleagues.
A statement the morning after the death read: 'It is with great sadness that we were informed this morning of the death of our colleague Helric Fredou, assigned as Deputy Director of the Regional Service Judicial Police in Limoges.'
'On this particular day of national mourning, police commissioners are new both hit hard by the tragic death of one of their own.'
'The Union of Commissioners of the National Police would like to present its most sincere condolences to the relatives of Helric.'